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target dna sequences  (Thermo Fisher)


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    Structured Review

    Thermo Fisher target dna sequences
    Target Dna Sequences, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/target+dna+sequence/DNA/pmc12365741-73-0-45
    Average 99 stars, based on 1 article reviews
    target dna sequences - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Cloning, Characterization, and Computer-Aided Evolution of a Thermostable Laccase of the DUF152 Family From Klebsiella michiganensis.
    Article Snippet: .. First, the target DNA sequence was designed according to the codon usage of E. coli with the online tool of NovoPro and the corresponding DNA fragment was chemically synthesized by Thermo Fisher Scientific. ..

    Article Title: Protein arginine methyltransferase 7 linked to schizophrenia through regulation of neural progenitor cell proliferation and differentiation.
    Article Snippet: .. Subsequently, the plasmid with the insertion of target DNA sequence or the empty vector plus the pRLTK, serving as an internal reference, is separately transferred into cells seeded in the 24-well plate using Lipofectamine 3000 (Thermo Fisher). .. After 24 h of transfection, the Firefly and Renilla luciferase activities are successively measured following the manufacturer’s instruction (Promega).

    Article Title: Genetic Variability of Loci Affecting Meat Quality and Production in Nero Siciliano Pig Breed
    Article Snippet: .. The target DNA sequence for each locus was amplified starting from 50 ng/μL of genomic DNA in a total reaction volume of 50 μL using the MiniAmp Plus thermal cycler (Applied Biosystems, Foster City, CA, USA). .. The PCR products were purified using Exo-SAP digestion and then dideoxy sequencing was performed using the BigDyeTM Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA).

    Article Title: HiBC: a publicly available collection of bacterial strains isolated from the human gut.
    Article Snippet: P. vulgatus total genomic and plasmid DNA was extracted using the Mericon DNA Bacteria Plus Kit following the manufacturer’s instructions (Qiagen). .. This DNA was used as a template to amplify the target DNA sequence using Thermo Fisher Scientific Phusion Hot Start 2 DNA polymerase and the above primer set. .. The amplified DNA was visualised by gel electrophoresis to confirm the expected DNA size, and the DNA was extracted and purified using Thermo Fisher Scientific GeneJET gel extraction kit.

    Article Title: Genetic Variability of Loci Affecting Meat Quality and Production in Nero Siciliano Pig Breed.
    Article Snippet: .. The target DNA sequence for each locus was amplified starting from 50 ng/μL of genomic DNA in a total reaction volume of 50 μL using the MiniAmp Plus thermal cycler (Applied Biosystems, Foster City, CA, USA). .. The PCR products were purified using Exo-SAP digestion and then dideoxy sequencing was performed using the BigDyeTM Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA).

    Article Title: CRISPR-Mediated SRY Gene Mutation Increases the Expression of Female Lineage-Specific Gene in Pre-Implantation Buffalo Embryo.
    Article Snippet: In mammals, sex determination is governed by the SRY gene on the Y chromosome, redirecting gonadal development from forming ovaries to testes.. Mutations or alterations in the SRY gene can significantly affect phenotypic changes and lineagespecific markers.. This study aims to elucidate the role of the SRY gene in buffalo embryos using CRISPRCas9 technology.

    Article Title: Cloning, Characterization, and Computer‐Aided Evolution of a Thermostable Laccase of the DUF152 Family From Klebsiella michiganensis
    Article Snippet: .. First, the target DNA sequence was designed according to the codon usage of E. coli with the online tool of NovoPro and the corresponding DNA fragment was chemically synthesized by Thermo Fisher Scientific. ..

    Article Title: HiBC: a publicly available collection of bacterial strains isolated from the human gut
    Article Snippet: P. vulgatus total genomic and plasmid DNA was extracted using the Mericon DNA Bacteria Plus Kit following the manufacturer’s instructions (Qiagen). .. This DNA was used as a template to amplify the target DNA sequence using Thermo Fisher Scientific Phusion Hot Start 2 DNA polymerase and the above primer set. .. The amplified DNA was visualised by gel electrophoresis to confirm the expected DNA size, and the DNA was extracted and purified using Thermo Fisher Scientific GeneJET gel extraction kit.

    Synthesized:

    Article Title: Cloning, Characterization, and Computer-Aided Evolution of a Thermostable Laccase of the DUF152 Family From Klebsiella michiganensis.
    Article Snippet: .. First, the target DNA sequence was designed according to the codon usage of E. coli with the online tool of NovoPro and the corresponding DNA fragment was chemically synthesized by Thermo Fisher Scientific. ..

    Article Title: Cloning, Characterization, and Computer‐Aided Evolution of a Thermostable Laccase of the DUF152 Family From Klebsiella michiganensis
    Article Snippet: .. First, the target DNA sequence was designed according to the codon usage of E. coli with the online tool of NovoPro and the corresponding DNA fragment was chemically synthesized by Thermo Fisher Scientific. ..

    Plasmid Preparation:

    Article Title: Protein arginine methyltransferase 7 linked to schizophrenia through regulation of neural progenitor cell proliferation and differentiation.
    Article Snippet: .. Subsequently, the plasmid with the insertion of target DNA sequence or the empty vector plus the pRLTK, serving as an internal reference, is separately transferred into cells seeded in the 24-well plate using Lipofectamine 3000 (Thermo Fisher). .. After 24 h of transfection, the Firefly and Renilla luciferase activities are successively measured following the manufacturer’s instruction (Promega).

    Amplification:

    Article Title: Genetic Variability of Loci Affecting Meat Quality and Production in Nero Siciliano Pig Breed
    Article Snippet: .. The target DNA sequence for each locus was amplified starting from 50 ng/μL of genomic DNA in a total reaction volume of 50 μL using the MiniAmp Plus thermal cycler (Applied Biosystems, Foster City, CA, USA). .. The PCR products were purified using Exo-SAP digestion and then dideoxy sequencing was performed using the BigDyeTM Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA).

    Article Title: Genetic Variability of Loci Affecting Meat Quality and Production in Nero Siciliano Pig Breed.
    Article Snippet: .. The target DNA sequence for each locus was amplified starting from 50 ng/μL of genomic DNA in a total reaction volume of 50 μL using the MiniAmp Plus thermal cycler (Applied Biosystems, Foster City, CA, USA). .. The PCR products were purified using Exo-SAP digestion and then dideoxy sequencing was performed using the BigDyeTM Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA).

    Generated:

    Article Title: CRISPR-Mediated SRY Gene Mutation Increases the Expression of Female Lineage-Specific Gene in Pre-Implantation Buffalo Embryo.
    Article Snippet: In mammals, sex determination is governed by the SRY gene on the Y chromosome, redirecting gonadal development from forming ovaries to testes.. Mutations or alterations in the SRY gene can significantly affect phenotypic changes and lineagespecific markers.. This study aims to elucidate the role of the SRY gene in buffalo embryos using CRISPRCas9 technology.

    Polymerase Chain Reaction:

    Article Title: CRISPR-Mediated SRY Gene Mutation Increases the Expression of Female Lineage-Specific Gene in Pre-Implantation Buffalo Embryo.
    Article Snippet: In mammals, sex determination is governed by the SRY gene on the Y chromosome, redirecting gonadal development from forming ovaries to testes.. Mutations or alterations in the SRY gene can significantly affect phenotypic changes and lineagespecific markers.. This study aims to elucidate the role of the SRY gene in buffalo embryos using CRISPRCas9 technology.



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